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UV-CD12 beamline

UV-CD12 is a high flux vacuum ultraviolet/ultraviolet beamline for steady-state and time-resolved synchrotron radiation circular dichroism (SRCD). SRCD can be used to investigate secondary structure of proteins and other macromolecules.

The high flux and wide wavelength range of the synchrotron radiation allows a wide range of experiments, for example:

  • Determination of secondary structure of proteins that cannot be crystallised.
  • Investigation of the effects of the binding of ligands (e.g. drugs) on protein secondary structure.
  • Study of dynamic processes such as protein folding.
  • Studies of the effects of crystallisation media on protein structure.
  • Determination of the secondary structure content of membrane proteins.
  • Study of enzyme active site changes.
  • Investigation of carbohydrate structure


The UV-CD12 beamline was conceived and designed by the Centre for Protein and Membrane Structure and Dynamics (CPMSD), a consortium of U.K. structural biologists. It was constructed at the SRS synchrotron facility of the Daresbury Laboratory and opened for users in 2003. Following the closure of SRS in August 2008, UV-CD12 was donated to ANKA in order to continue its working life.

ANKA is proud to have been selected to receive UV-CD12 against strong international competition, and gratefully acknowledges the U.K. Science and Technology Facilities Council for the gift of this excellent beamline.

UV-CD12 Anlage

In the original UV-CD12 design at Daresbury, a plane mirror 4.1 m from the source deflects the beam vertically upwards through the storage ring roof into an experimental cabin on the upper floor. Here the beam enters the monochromator from below and is reflected back to the horizontal by the toroidal grating located 3 m above the ring plane. ANKA has no upper floor, but a near duplication of the layout could be achieved by shortening the vertical arm of the optical path to 1.2 m and lengthening the horizontal arm to 5.9 m. This brings the experimental hutch down to elevated ground floor level while preserving the 7.1 m total focus distance from source to toroidal grating. At the same time, the 45% increased distance from source to extraction mirror compensates for the additional heat load caused by ANKA’s higher electron beam energy (2.5 versus 2.0 GeV).

 

Layout UV CD 12

Source 1.5 T Bending magnet (Ec= 6 keV)
Beam divergence 30.8 mrad (horz) x 3.7 mrad (vert)
Optics Planar water-cooled mirror, Al-coated silicon
Toroidal holographic grating, Al-coated silica
Photon flux ∼ 1012 photon/s/nm bandwidth at 200 nm
Wavelength range 130 – 340 nm
Energy range 3.6 – 9.5 eV
Wavelength resolution 0.5 to 2.0 nm
Photoelastic modulator CaF2, 50 kHz (Hinds Instruments PEM-90)
Detector Electron Tubes 9406B or solar blind PM (ET 9402B)
Journal papers UV-CD12
Title Author Source Date Link

Yates,EA; Terry,CJ; Rees,C; Rudd,TR; Duchesne,L; Skidmore,MA; Levy,R; Thanh,NTK; Nicholas,RJ; Clarke,DT; Fernig,DG;

Biochemical Society Transactions; Vol. 34; pp 427-430;

2006

Wien,F; Miles,AJ; Lees,JG; Hoffmann,S; Wallace,BA;

Journal of Synchrotron Radiation; Vol. 12; pp 517-523;

2005

Wien,F; Miles,AJ; Lees,J; Cuff,AL; Janes,RW; Wallace,BA;

Biophysical Journal; Vol. 88 part 2; pp 554a-554a;

2005

Whitmore,L; Wallace,BA;

Nucleic Acids Research; Vol. 32; pp 668-673;

2004

Whitmore,L; Janes,RW; Wallace,BA;

Chirality; Vol. 18 (6); pp 426-429;

2006

Wallace,BA; Wien,F; Stone,TC; Miles,AJ; Lees,JG; Janes,RW;

Biophysical Journal; Vol. 90;

2007

Wallace,BA; Wien,F; Miles,AJ; Lees,JG; Hoffman,SV; Evans,P; Wistow,GJ; Slingsby,C;

Faraday Discussions; Vol. 126; pp 237-243;

2004

Wallace,BA; Whitmore,L; Janes,RW;

Proteins Structure, Function and Bioinformatics; Vol. 62 (1); pp 1-3;

2006

Wallace,BA; Lees,JG; Orry,AJW; Lobley,A; Janes,RW;

Protein Science; Vol. 12; 4; pp 875-884;

2003

Wallace,BA; Janes,RW; Orry,A;

Biophysical Journal; Vol. 80;

2001

Wallace,BA; Janes,RW;

Biochemical Society Transactions; Vol. 31; 3; pp 631-633;

2003

Wallace,BA;

Nature Structural Biology; Vol. 7; pp 708-709;

2000

Wallace,BA;

Austrailian Biochemist; Vol. 36; pp 47-50;

2005

Wallace,BA;

Journal of Synchrotron Radiation; Vol. 7; pp 289-295;

2000

Tew,DJ; Bottomley,SP; Smith,DP; Ciccotosto,GD; Babon,J; Hinds,MG; Masters,CL; Cappai,R; Barnham,KJ;

Biophysical Journal; Vol. 94; pp 2752-2766;

2008

Stanley,WA; Sokolova,A; Brown,A; Clarke,DT; Wilmanns,M; Svergun,DI;

Journal of Synchrotron Radiation; Vol. 11; pp 490-496;

2004

Skidmore,MA; Dumax-Vorzet,AF; Guimond,SE; Rudd,TR; Edwards,EA; Turnbull,JE; Craig,AG; Yates,EA;

Journal of Medicinal Chemistry; Vol. 51; 5; pp 1453-1458;

2008

Scott,DJ; Grossman,JG; Tames,JRH; Byron,O; Wilson,KS; Otto,BR;

Journal of Molecular Biology; Vol. 315; pp 1179-1187; isbn=5;

2002

S. Ruskamo, R.P. Yadav, S. Sharma, M. Lehtimäki, S. Laulumaa, S. Aggarwal, M. Simons, J. Bürck , A.S. Ulrich, A.H. Juffer, I. Kursula, P. Kursula

Acta Crystallogr. D 70 1, 165-176

2014

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Rudd,TR; Skidmore,MA; Guimond,SE; Guerrini,M; Cosentino,C; Edge,R; Brown,A; Clarke,DT; Torri,G; Turnbull,JE; Nichols,RJ; Fernig,DG; Yates,EA;

Carbohydrate Research; Vol. 343; 12; pp 2184-2193;

2008

Rudd,TR; Nichols,RJ; Yates,EA;

Journal of the American Chemical Society; Vol. 130; 7; pp 2138-2139;

2008

Rudd,TR; Guidmond,SE; Skidmore,MA; Duchesne,L; Guerrini,M; Torri,G; Cosentino,C; Brown,A; Clarke,DT; Turnbull,JE; Fernig,DG; Yates,EA;

Glycobiology; Vol. 17; pp 983-993;

2007

Richards,MW; Easton,JT; Butcher,AJ; Berrow,NS; Dolphin,AC; Perkins,SJ; Wallace,BA;

Biophysical Journal; Vol. 84; pp 136a-?;

 

2003

Pinotsis,N; Pethoukov,M; Lange,S; Svergun,D; Zou,P; Gautel,M; Wilmanns,M;

Journal of Structural Biology; Vol. 155; 2; pp 239-250;

2006

Pinotsis,N; Lange,S; Perriard,JC; Svergun,DI; Wilmanns,M;

EMBO Journal; Vol. 27; pp 253-264;

2008

Nicholson,JM; Wood,CM; Reynolds,CD; Brown,A; Lambert,SJ; Chantalat,L; Baldwin,JP;

Annals of the New York Academy of Sciences; Vol. 1030; pp 642-653;

2004

N.J. Harris, H.E. Findlay, J. Simms, X. Liu, P.J. Booth

J. Mol. Biol. 426 7, 1812-1825

2014

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N. D´Avanzo, E. C. McCusker, A. M. Powl, A. J. Miles, C. G. Nichols, B. A. Wallace 

PLoS ONE 8, e61216 

2013 PDF

Miles,AJ; Wien,F; Wallace,BA;

Analytical Biochemistry; pp 338-339;

2004

Miles,AJ; Wien,F; Lees,JG; Wallace,BA;

Spectroscopy; Vol. 19; pp 43-51;

2005

Miles,AJ; Wien,F; Lees,JG; Rodger,A; Janes,RW; Wallace,BA;

Biophysical Journal; Vol. 86; pp 358a-?;

2004

Miles,AJ; Wien,F; Lees,JG; Rodger,A; Janes,RW; Wallace,BA;

Spectroscopy; Vol. 17; pp 653-661;

 

2003

Miles,AJ; Whitmore,L; Wallace,BA;

Protein Science; Vol. 14; pp 368-374;

2005

Miles,AJ; Wallace,BA;

Chemical Society Reviews; Vol. 35; pp 39-51;

2006

Miles,AJ; Wallace,BA;

Chemical Society Reviews; Vol. 35 (1); pp 39-51;

2005

Miles,AJ; Janes,RW; Brown,A; Clarke,DT; Sutherland,JC; Tao,Y; Wallace,BM; Hoffmann,SV;

Journal of Synchrotron Radiation; Vol. 15; pp 420-422;

2008

Miles,AJ; Hoffman,SV; Tao,Y; Janes,RW; Wallace,BA;

Newbeamlines and new applications in biology
Spectroscopy; Vol 21; pp 245-255;

2007

Miles,AJ; Drechsler,A; Kristan,K; Anderluh,G; Norton,RS; Wallace,BA; Separovic,F;

Biochim Biophys Acta (Biomembranes); Vol. 1778; pp 2091-2096;

2008

McKibbin,C; Farmer,NA; Jeans,C; Reeves,PJ; Khorana,HG; Wallace,BA; Edwards,PC; Villa,C; Booth,PJ;

Journal of Molecular Biology; Vol. 374; 5; pp 1319-1332;

2007

McCabe,RW; Rodger,A; Taylor,A;

Enzyme and Microbial Technology; Vol. 36; pp 70-74;

2005

Manolopulos,S; Clarke,D; Derbyshire,G; Jones,GR; Read,P; Torbet,M;

Nuclear Instruments and Methods in Physics Research A; Vol. 510; pp 406-409;

2003

Manolopoulos,S; Clarke,DT; Derbyshire,GE; Jones,GR; Read,P; Torbet,M;

Nuclear Instruments and Methods in Physics Research A; Vol. A531; pp 302-306;

2004

M.B. Ulmschneider, J.P. Ulmschneider, N. Schiller, B.A. Wallace, G. von Heijne, S.H. White

Nat. Commun. 5, 4863-

2014

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M. Myllykoski, A. Raasakka, M. Lehtimäki, H. Han, I. Kursula, P. Kursula 

J. Mol. Biol. 425, 4307-4322 

2013 PDF

Lees,JG; Wallace,BA;

 Spectroscopy; Vol. 16; pp 121-125;

2002

Lees,JG; Smith,BR; Wien,F; Miles,AJ; Wallace,BA;

Analytical Biochemistry; Vol. 332; pp 285-289;

2004

Lees,JG; Miles,AJ; Wien,F; Wallace,BA;

Bioinformatics; Vol. 22 (16); pp 1955-1962;

2006

Lees,JG; Miles,AJ; Janes,RW; Wallace,BA;

BMC Bioinformatics; Vol. 7; pp 507-517;

2006

Lau,TL; Ambroggio,EE; Tew,DJ; Cappai,R; Masters,CL; Fidelio,GD; Barnham,KJ; Separovic,F;

Journal of Molecular Biology; Vol. 356; 3; pp 759-770;

2006

Lau,TL; Ambroggio,EE; Tew,DJ; Cappai,R; Masters,CL; Fidelio,DG; Barnham,KJ; Separovic,F;

Journal of Molecular Biology; Vol. 356; pp 759-770;

2005

Jones,GR; Clarke,DT;

Faraday Transactions; Vol. 126; pp 223-236;

2004

Janes,RW; Cuff,AL;

Journal of Synchrotron Radiation; Vol. 12; 4; pp 524-529;

2005

J.L.S. Lopes, T.M. Nobre, E.M. Cilli, L.M. Beltramini, A.P.U. Araújo, B.A. Wallace

Biochim. Biophys. Acta-Biomembr. 1838 12, 3145-3152

2014

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J. L. S. Lopes, D. Orcia, A. P. U. Araujo, R. DeMarco, B. A. Wallace 

Biophys. J. 104, 2512-2520 

2013 PDF

J. Bürck, S. Roth, A.S. Ulrich

18th Hiroshima International Symposium on Synchrotron Radiation

2014

Gales,L; Cortes,L; Almeida,C; Melo,CV; do Carmo Costa,M; Maciel,P; Clarke,DT; Damas,AM; Macedo-Ribeiro,S; (2005)

Journal of Molecular Biology; Vol. 353; 3; pp 642-654;

2005

G. Muruganandam, J. Bürck, A. S. Ulrich, I. Kursula, P. Kursula 

J. Phys. Chem. B 117, 14983-14993 

2013 PDF

Flavell,WR; Seddon,EA; Weightman,P; Chesters,MA; Poole,MW; Quinn,FM; Clarke,DT; Clarke,JA; Tobin,MJ;

Journal of Physics - Condensed Matter; pp s2405-s2412;

2004

Evans,P; Wyatt,K; Wistow,GJ; Bateman,OA; Wallace,BA; Slingsby,C;

Journal of Molecular Biology; Vol. 343; 2; pp 435-444;

2004

Evans,P; Bateman,OA; Slingsby,C; Wallace,BA;

Research; Vol 84; pp 1001-1008;

2007

Drechsler,A; Miles,AJ; Norton,RC; Wallace,BA; Separovic,F;

European Biophysics Journal; Vol 39; pp 121-127;

2009

Doig,AJ; Cochran,DAE; Hughes,E; Penel,S; Sun,JK; Stapley,BJ; Clarke,DT; Jones,GR;

Biochimica et Biophysica Acta; Vol. 68; 95; pp 95-110;

2001

D. Windisch, C. Ziegler, J. Bürck, A. S. Ulrich

Biol. Chem. 395 12, 1443-1452

2014

PDF

Curnow,P; Booth,PJ;

Proceedings of the National Academy of Sciences USA; Vol. 104; pp 18970-18975;

2007

Cronin,NB; O'Reilly,A; Duclohier,H; Wallace,BA;

Biochemistry; Vol. 44; 2; pp 441-449;

2005

Cronin,N; O'Reilly,A; Duclohier,H; Wallace,BA;

Biophysical Journal; Vol. 86; pp 168a-?;

2004

Cowieson,NP; Miles,AJ; Robin,G; Forwood,JK; Kobe,B; Martin,JL; Wallace,BA;

Proteins Structure, Function and Bioinformatics; Vol. 70; pp 1142-1146;

2008

Clarke,DT; Jones,G;

Journal of Synchrotron Radiation; Vol. 11; pp 142-149;

2004

Clarke,DT; Bowler,MA; Fell,BD; Flaherty,JV; Grant,AF; Jones,GR; Martin-Fernandez,ML; Shaw,DA; Todd,B; Wallace,BA; Towns-Andrews,E;

Synchrotron Radiation News; Vol. 13; pp 21-27;

2000

Ch. Wang, U. Neugebauer, J. Bürck, M. Myllykoski, P. Baumgärtel, J. Popp, P. Kursula 

PLoS ONE 6, e19915 

2011

Cerasoli,E; Kelly,SM; Coggins,JR; Boam,DJ; Clarke,DT; Price,NC;

European Journal of Biochemistry; Vol. 269; 8; pp 2124-2132;

2002

Bulheller,B; Miles,AJ; Wallace,BA; Hirst,J;

Journal of Physical Chemistry B; Vol. 112; pp 1866-1874;

2008

Barnham,KJ; Kenche,VB; Ciccotosto,GD; Smith,DP; Tew,DJ; Liu,X; Perez,K; Cranston,GA; Johanssen,TJ; Volitakis,I; Bush,AI; Masters,CL; White,AR; Smith,JP; Cherny,RA; Cappai,R;

Proceedings of the National Academy of Sciences USA; Vol. 105; pp 6813-6818

2008

Andrew,CD; Warwicker,J; Jones,GR; Doig,AJ;

Biochemistry; Vol. 41; 6; pp 1897-1905;

2002

Andrew,CD; Penel,S; Jones,GR; Doig,AJ;

Structure Function and Genetics; Vol. 45; 4; pp 449-455;

2001

A. Szambowska, I. Tessmer, P. Kursula, C. Usskilat, P. Prus, H. Pospiech, F. Grosse

Nucleic Acids Res. 42 4, 2308-2319

2014

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