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Clouding of Eye Lenses: New Hope for Cataracts Patients

Clouding of Eye Lenses: New Hope for Cataracts Patients
Date: May 2016

Trace elements are essential for normal development and vital for survival of an organism. The trace element iron that is part of haemoglobin is able to bind oxygen and ensures its transport to the body cells via ion transporters. During times of environmental stress, e.g. UV or heat exposure, the level of chemically reactive molecules containing oxygen increases dramatically in the human body. Normal cells are able to neutralize this so called oxygen stress. Some mutated cells have lost this ability.

In humans, mutations in certain iron storage proteins and iron exporters lead to hyperferritinemia-cataract syndrome. This genetic disorder causes progressive dimming and blurriness of vision.

Using X-ray fluorescence (μ-XRF) imaging, KIT scientists were able to examine inorganic element distribution in zebrafish embryonic eyes. Many elements were highly enriched in the pigment epithelial (PE) layer of the zebrafish embryo. By inhibiting mature melanosome formation, detected element levels and enrichment in the PE could be reduced.

These studies suggest that melanosomes in pigment epithelial cells protect the lens from oxidative stress during embryonic development, likely by buffering trace elements.

Localization of elements in the eye
X-ray fluorescence images of the eye of a 3-dpf embryo: Localization of enriched elements in the RPE (arrows).

Masanari Takamiya, Feng Xu, Heikki Suhonen, Victor Gourain, Lixin Yang, Nga Yu Ho, Lukas Helfen, Anne Schröck, Christelle Etard, Clemens Grabher, Sepand Rastegar, Günther Schlunck, Thomas Reinhard, Tilo Baumbach & Uwe Strähle, “Melanosomes in pigmented epithelia maintain eye lens transparency during zebrafish embryonic development”, Scientific Reports 6 (2016)